FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Scrotal circumference, Trypanosoma brucei brucei, Trypanosoma evansi, Yankasa rams
The effects of trypanosomosis on the scrotal circumferences and semen characteristics of Yankasa rams were studied for ninety eight days (14 weeks). Sixteen Yankasa rams aged between 24 to 30 months and weighed between 22 to 25 kg were acclimatized for a period of two months in a clean fly- proof house and were adequately fed and given water ad libitum. Of the sixteen rams, twelve that were clinically fit for the experiment at the end of the acclimatization were randomLy divided into four Groups: I, II, III and IV, of three rams each. Groups I and II were each challenged singly with experimental T. brucei brucei (Federe strain) and T. evansi (Sokoto strain) respectively, while Group III was challenged with mixed T. brucei brucei and T. evansiparasites (50% of each species in the infective inoculum) and Group IV was left as uninfected control. Each infected ram received 2 mL containing 2 x 106 trypomastigotes via the jugular vein. All the infected groups developed clinical signs typical of trypanosomosis at various prepatent periods, with significant (P<0.05) decrease in values of haematological indices. There was a drastic and significant decrease in scrotal circumferences in the infected rams with significant deterioration (P<0.05) in semen quality of all the infected rams, characterized by decrease in volume or cessation of semen production, a significant (P<0.05) decrease in progressively motile sperm, elevated numbers of dead sperm and significant increase (P<0.05) in spermatozoa morphological abnormalities in all the infected rams. The rams especially in Groups I and III were all deemed unfit for breeding by the end of the 98 days post infection. However, the uninfected rams were healthy and had normal semen characteristics throughout the study period. The results indicate that trypanosomosis due to experimental T. b. brucei or T.evansi or Mixed infections (of both parasites) caused significant decrease in scrotal circumference and severe deterioration in semen quality with resultant infertility in Yankasa rams.
Alabi JF 2005. Effects of Energy Supplementation on Growth And Reproductive Function of Bunaji and Friesian X Bunaji Bulls. Ph.D. Dissertation, Ahmadu Bello University, Zaria. Anosa VO & Isoun TT 1974. Pathology of experimental Trypanosoma vivax infection in sheep and goats. Zbl Vet Me.,, 30 (1): 685 – 700. Bandyopaghy SK, Bhattacharyya B, Choudhury RR & Basu S 2007. Textbook of Veterinary Gynaecology, Artificial Insemination, Obstetrics and Assisted Reproduction. Kalyani Publishers, New Delhi, Second Edition, p. 125. Bitto II, Akusu MO, Egbunike GN & Akpokodje JU 2000. The physical characteristics of ovine-caprine ejaculate mixtures and the survival of their spermatozoa in cow’s milk extender in the humid tropics. Trop. J. Anim. Sci., 3(1): 147 – 152. Biryomumaisho S, Rwakishaya EK, Melville SE, Cailleau A & Lubega GW 2013. Livestock trypanosomosis in Uganda: Parasite heterogeneity and anaemiastatus of naturally infected cattle, goats and pigs. Parasitol. Res., 112(4): 1443 – 1450. Blom E 1972. The ultrastructure of some characteristic sperm defects and a proposal for a new classification of the bull spermiogram. Atti del VIII. Simposio Int. di Zootechnia. Milan, pp. 125 – 139. Bossard G, Boulange A, Holzmuller P, Thevenon S, Patrel D. & Authie E 2010. Serodiagnosis of bovine trypanosomosis based on HSP70/BiP inhibition ELISA. Vet. Parasitol., 173(1-2): 39 – 47. Egbuka DO 1995. Effects of male presence and mounting on the induction of ovulation and success rate of artificial insemination in rabbits. B. Agric. Tech. Project Report, Federal University of Technology, Owerri. Herbert WJ & Lumsden WHR 1976. Trypanosoma brucei: A rapid "matching" method for estimation of the host parasitaemia. Exp. Parasitol., 40(3): 427 – 431. Hoare CA 1972. Trypanosomes of mammals: A Zoological Monograph. Oxford and Edinburgh, pp. 1 – 3. Ikede BO & Akpavie SO 1982. Delay in resolution of trypanosome induced genital lesions in male rabbit infected with Trypanosoma brucei and treated with Diminazene aceturate. Res. Vet. Sci., 32: 374 – 376. Liebenehm S 2008. Economic impact of livestock research on farmer’s knowledge and productivity: The case of trypanosomosis in West Africa. MSc Thesis. Leibniz University of Hannover, Germany. Michael AJ, Alexopoulos C, Pontiki EA, Hadjipavlou-Litina DJ, Saratsis P, Ververidis HN & Boscos CM 2009. Effect of antioxidant supplementation in semen extenders on semen quality and reazctive oxygen species of chilled canine spermatozoa. Anim. Rep. Sci., 112: 119 – 135. Morar IA, Groza IS, Borzan M, Emike P, Neagu VR, Pop ALR, Mate C & Emilia G 2010. Studies regarding collection, evaluation and conservation of European mouflon (Ovis ammon musimon) Semen. Lucrări Stiinłifice Medicină Veterinară, 2: 15 – 24. Ogbaje CI, Lawal IA & Ajanusi OJ 2011. Infectivity and pathogenicity of Sokoto (northern Nigeria) isolate of Trypanosoma evansi in West African Dwarf Goats. Int. J. Anim. & Vet. Adv., 3(3): 117 – 124. Ogunlade JT, Ewuola EO, Gbore FA, Bandyopadhyay R, Niezen J & Egbunike GN 2006. Testicular and epididymal sperm reserves of rabbits fed fumonisin contaminated diets. World Applied Sci. J., 1(1): 35 – 38. Okubanjo OO, Sekoni VO, Nok AJ, & Adeyeye AA 2014. Testicular and epididymal pathology in Yankasa rams experimentally infected with Trypanosoma congolense. Asian Pac. J. Trop. Dis., 4(3): 185 – 189. Omalaka EEO 1992. The Effect of Time and Frequency of Collection on the Quantity and Quality of Boar Semen. Bachelor of Agricultural Technology Project Report, Department of Animal Science and Technology, Federal University of Technology, Owerri. Ouma EM 2010. Management of trypanocidal drug resistance in cattle in identified chemo-resistance hot spots in the administrative district of Sikasso, South-East Mali. Ph.D Thesis. Freie Universitat Berlin, Germany. Osaer S, Goossens B, Sauveroche B & Dempfle L 1997. Evaluation of the semen quality and reproductive performance of Trypano-tolerant Djallonke rams following an artificial infection with Trypanosoma congolense. Small Rum Res., 24: 213 – 222. Rao AR 1971. Changes in the morphology of sperm during their passage through genital in bull abnormal and impaired spermatogenesis. Ph.D. Thesis. Stockholm. Rekwot PI, Oyedipe EO & Ehoche GW 1994. The effects of feed restriction and realimentation on the growth and reproductive function of Bokoloji bulls. Theriogenology, 42: 287 – 295. Sekoni VO, Gustafsson BK & Mather EC 1981. Influence of wet fixation, staining techniques and storage time on bulls sperm morphology. Nordisk. Vet. Med., 33: 161 – 166. Sekoni VO, Njoku CO., Kumi-Diaka J & Saror DI 1990. Pathological changes in male genitals of cattle infected with Trypanosome vivax and Trypanoosoma congolense. Brit. Vet. J., 146 – 175. Sekoni VO 1994. Reproductive disorders caused by animal Trypanosomiasis: A review. Theriogenology, 42: 557 570. Shehu SA, Ibrahim NDG, Esievo KAN & Mohammed G. 2010. Study on the infectivity of Trypanosoma evansi isolate in Savannah Brown Bucks. J. Vet. Sci., 8(1&2): 1 – 3. Silva TM, Olinda RG, Rodrigues CM, Camara AC, Lopes FC, Coelho WA, Ribeiro M.F., Freitas C, Teixeira, MM & Batista JS 2013. Pathogenesis of reproductive failure induced by Trypanosoma vivax in experimentallyinfected pregnant ewes. Vet. Res., 44(1): 1 – 5. Steel RG & Torrie JH 1980. Principles and Procedures of Statistics. An Approach. 2nd ed. McGraw-Hill Book Co. Inc. New York. Wilson RT & Durkin JW 1984. Age at permanent incisor eruption in indigenous goats and sheep in semi-arid Africa. Small Rum & Cam. Grp. Group Document No. SR C3, pp. 451– 455. Woo PTK 1969. The haematocrit centrifuge technique for the detection of trypanosomes in blood. Can. J. Zool., 47: 921 – 923. Zemjanis R 1977. Collection and evaluation of semen. In: Diagnostic and Therapeutic Techniques in Animal Reproduction. The Williams and Wilkins Co. Baltimore, p. 242
